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Registros recuperados : 126 | |
1. | | REYNO, R. Paspalum notatum TB42. In: INIA TACUAREMBÓ. JORNADA DE DIVULGACIÓN, 6 DE FEBRERO, 2015, TAMBORES, URUGUAY. Manejo de la fertilización de pasturas, forrajes y campo natural bajo riego suplementario. Tacuarembó (Uruguay): INIA, 2015. p. 41-44 (Serie Actividades de Difusión; 742)Biblioteca(s): INIA Tacuarembó. |
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8. | | REAL, D.; REYNO, R. Mejoramiento genético de leguminosas forrajeras para áreas extensivas Revista INIA, 2006, no. 9, p. 24-25 Agradecimientos: Los autores agradecen a los Téc. Agrop. Mauro Zarza, Rúben Mérola y Ana Viana por su fundamental trabajo en el desarrollo de este proyecto, a los técnicos de INIA Marco Dalla Rizza y Nora Altier, a los técnicos del...Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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18. | | FRANCO, R.; JAURENA, M.; REYNO, R. Effect of sowing density on the productivity of a native grassland restored with Bromus auleticus. [Efecto de la densidad de siembra en la productividad de un campo natural restaurado con Bromus auleticus]. [Efeito da densidade de semeadura na productividade de um campo nativo restaurado com Bromus auleticus]. Plant production. Agrociencia Uruguay, 2021, vol. 25, n.2, article e333. DOI: https://doi.org/10.31285/AGRO.25.333 Article history: Received 25 Feb 2020; Accepted 01 Jul 2021; Published 06 Sep 2021.
Editor: Sebastián Mazzilli, Universidad de la República Paysandú, Uruguay.
Correspondence: Rafael Reyno, mail: rreyno@inia.org.uyBiblioteca(s): INIA Las Brujas. |
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Registros recuperados : 126 | |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha actual : |
21/02/2014 |
Actualizado : |
22/01/2020 |
Tipo de producción científica : |
Tesis |
Autor : |
REYNO, R. |
Afiliación : |
RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Improving acid and aluminum tolerance in alfalfa using breeding and genomics : tesis Ph.D. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
Athens (USA): University of Georgia, 2012. |
Páginas : |
116 p. |
Idioma : |
Inglés |
Contenido : |
Alfalfa, Medicago sativa L., is an important forage crop in the U.S. and worldwide. However, in acid soil, its productivity and persistence dramatically decrease. Acid-soil syndrome causes a severe toxicity that inhibits root growth and development exacerbated by Al+3. Breeding and genomic approaches to improve acid/Al tolerance provide new opportunities to grow alfalfa in lands considered marginal for alfalfa growth. In this study, three different approaches were used to identify and to evaluate acid/Al tolerance in alfalfa: (1) identification and mapping of quantitative trait loci (QTL) associated with acid/Al tolerance, (2) evaluation of transgenic plants over-expressing Pseudomonas aeruginosa citrate synthase (CS) and/or a gene coding for a plasma membrane H+-ATPase from Daucus carota (DcPA1), and (3) comparison of genetic gain for acid/Al tolerance as assessed by seedling biomass production in acidic soil under greenhouse conditions. Single-factor analysis and interval mapping identified QTL for Al tolerance on Altet-4 and on NECS-141 linkage groups. The phenotypic variation explained by individual QTL ranged from 9.5 to 35.3%. Some of these QTL were detected in multiple environments and for multiple traits while others were environment-specific. Four isogenic T2 populations, containing neither, one, or both transgenes (CS+DcPA1) were evaluated for acid/Al tolerance. The transgenic populations containing either CS or DcPA1 or both genes showed higher acid/Al tolerance than the non-transgenic population. No advantage of combining both transgenes in the same genetic background was observed. Lower levels of Al in shoot tissue were observed for the transgenic populations over the non-transgenic population. The comparison of selection methods showed that the phenotypic recurrent selection with gridding in unlimed soil was most effective at improving the Bulldog 805 population for shoot dry weight. This method of selection was the most effective in terms of the resources used and the responses achieved. Direct selection in unlimed soil resulted in increased alfalfa growth in acid and Al-rich soils compared to selection in limed soil. The information generated in these three experiments can be useful in improving our understanding of acid/Al tolerance in alfalfa and in developing cost-effective and efficient methods to obtain enhanced alfalfa germplasm in the future. MenosAlfalfa, Medicago sativa L., is an important forage crop in the U.S. and worldwide. However, in acid soil, its productivity and persistence dramatically decrease. Acid-soil syndrome causes a severe toxicity that inhibits root growth and development exacerbated by Al+3. Breeding and genomic approaches to improve acid/Al tolerance provide new opportunities to grow alfalfa in lands considered marginal for alfalfa growth. In this study, three different approaches were used to identify and to evaluate acid/Al tolerance in alfalfa: (1) identification and mapping of quantitative trait loci (QTL) associated with acid/Al tolerance, (2) evaluation of transgenic plants over-expressing Pseudomonas aeruginosa citrate synthase (CS) and/or a gene coding for a plasma membrane H+-ATPase from Daucus carota (DcPA1), and (3) comparison of genetic gain for acid/Al tolerance as assessed by seedling biomass production in acidic soil under greenhouse conditions. Single-factor analysis and interval mapping identified QTL for Al tolerance on Altet-4 and on NECS-141 linkage groups. The phenotypic variation explained by individual QTL ranged from 9.5 to 35.3%. Some of these QTL were detected in multiple environments and for multiple traits while others were environment-specific. Four isogenic T2 populations, containing neither, one, or both transgenes (CS+DcPA1) were evaluated for acid/Al tolerance. The transgenic populations containing either CS or DcPA1 or both genes showed higher acid/Al tolerance t... Presentar Todo |
Palabras claves : |
AMONG-WITHIN FAMILY SELECTION; CITRATE SYNTHASE; GREENHOUSE SOIL-BASED ASSAY; GRID SELECTION; PLASMA MEMBRANE H+-ATPASE; QUANTITATIVE TRAIT LOCI; RECURRENT SELECTION; TRANSGENIC; UNLIMED AND LIMED SOILS. |
Thesagro : |
ALFALFA; MEDICAGO SATIVA. |
Asunto categoría : |
A50 Investigación agraria |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3710/1/Reyno-Rafael-TESISphd.pdf
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Marc : |
LEADER 03154nam a2200253 a 4500 001 1019178 005 2020-01-22 008 2012 bl uuuu m 00u1 u #d 100 1 $aREYNO, R. 245 $aImproving acid and aluminum tolerance in alfalfa using breeding and genomics$btesis Ph.D. 260 $aAthens (USA): University of Georgia$c2012 300 $a116 p. 520 $aAlfalfa, Medicago sativa L., is an important forage crop in the U.S. and worldwide. However, in acid soil, its productivity and persistence dramatically decrease. Acid-soil syndrome causes a severe toxicity that inhibits root growth and development exacerbated by Al+3. Breeding and genomic approaches to improve acid/Al tolerance provide new opportunities to grow alfalfa in lands considered marginal for alfalfa growth. In this study, three different approaches were used to identify and to evaluate acid/Al tolerance in alfalfa: (1) identification and mapping of quantitative trait loci (QTL) associated with acid/Al tolerance, (2) evaluation of transgenic plants over-expressing Pseudomonas aeruginosa citrate synthase (CS) and/or a gene coding for a plasma membrane H+-ATPase from Daucus carota (DcPA1), and (3) comparison of genetic gain for acid/Al tolerance as assessed by seedling biomass production in acidic soil under greenhouse conditions. Single-factor analysis and interval mapping identified QTL for Al tolerance on Altet-4 and on NECS-141 linkage groups. The phenotypic variation explained by individual QTL ranged from 9.5 to 35.3%. Some of these QTL were detected in multiple environments and for multiple traits while others were environment-specific. Four isogenic T2 populations, containing neither, one, or both transgenes (CS+DcPA1) were evaluated for acid/Al tolerance. The transgenic populations containing either CS or DcPA1 or both genes showed higher acid/Al tolerance than the non-transgenic population. No advantage of combining both transgenes in the same genetic background was observed. Lower levels of Al in shoot tissue were observed for the transgenic populations over the non-transgenic population. The comparison of selection methods showed that the phenotypic recurrent selection with gridding in unlimed soil was most effective at improving the Bulldog 805 population for shoot dry weight. This method of selection was the most effective in terms of the resources used and the responses achieved. Direct selection in unlimed soil resulted in increased alfalfa growth in acid and Al-rich soils compared to selection in limed soil. The information generated in these three experiments can be useful in improving our understanding of acid/Al tolerance in alfalfa and in developing cost-effective and efficient methods to obtain enhanced alfalfa germplasm in the future. 650 $aALFALFA 650 $aMEDICAGO SATIVA 653 $aAMONG-WITHIN FAMILY SELECTION 653 $aCITRATE SYNTHASE 653 $aGREENHOUSE SOIL-BASED ASSAY 653 $aGRID SELECTION 653 $aPLASMA MEMBRANE H+-ATPASE 653 $aQUANTITATIVE TRAIT LOCI 653 $aRECURRENT SELECTION 653 $aTRANSGENIC 653 $aUNLIMED AND LIMED SOILS
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